Alexa Fluor dyes—Emission spectra. Figure 1. Emission spectra for Alexa Fluor dyes. Table 1. Properties of Alexa Fluor Dyes. * Typical emission color seen through the eyepiece of a conventional fluorescence microscope with appropriate filters. ** Extinction coefficient at lmax in cm–1M–1. *** Human vision is insensitive to light beyond ...405 Blue Brilliant Violet ™ 421 BD Horizon™ V450 VPD450 Pacific Blue™ Green BD Horizon V500 AmCyan Orange Brilliant Violet ™ 605a 375b Blue Hoechst 33342 BD Influx™ 488 Green FITC Alexa Fluor® 488 Yellow PE PI Orange BD Horizon PE-CF594 PE-Texas Red® Red 7-AAD PE-Cy5 PerCP PerCP-Cy5.5 Infrared PE-Cy7 532 or 561 …Vio® Dyes represent a family of fluorochromes for flow cytometry and fluorescence microscopy developed. by Miltenyi Biotec. VioBlue®, VioGreen™, Vio Bright FITC, PE-Vio® 615, PE-Vio 770, PerCP-Vio 700, Vio 515, Vio Bright 515, APC-Vio 770 are characterized by high fluorescence intensities and low spillover, making them an ideal choice for ... We have put together a comprehensive list of fluorochromes compatible with our MACSQuant Instruments. Multi-color flow cytometry and cell sorting applications require the use of a wide range of fluorochrome-conjugated antibodies and dyes. MACSQuant X, MACSQuant Analyzer 10, and MACSQuant Tyto® compatible dyes. Laser.Pacific Blue ; Pacific Green ; Pacific Orange ; Tetramethylrhodamine (TRITC) Texas Red (and Texas Red-X) PE, APC, related tandems APC-eFluor 780 ... Alexa Fluor 700 dye is a near-IR fluorophore used primarily for flow cytometry, optimally excited by the red laser. Photostability in buffer : Dim: 633-640: 720/30: 702: 723 : Relative staining ...DAPI (4′,6-diamidino-2-phenylindole) DAPI (4′,6-diamidino-2-phenylindole) is a blue-fluorescent DNA stain that exhibits ~20-fold enhancement of fluorescence upon binding to AT regions of dsDNA. It is excited by the violet (405 nm) laser line and is commonly used as a nuclear counterstain in fluorescence microscopy, flow cytometry, and ...Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ... We selected 7-hydroxycoumarin as our fluorophore for this study because its excitation and emission wavelengths are suitable for live-cell imaging (excitation 387–405 nm; emission 448 nm) , it is brighter than Enhanced Blue Fluorescent Protein (EBFP) [ε = 36,700 M-1 cm-1 and QY 0.7 for the anionic form , compared to ε = 31,500 M-1 cm-1 and ...Fluorophore Selection Guide. Fluorophores enable researchers to expand the limits of their multicolor experiments in high content analysis, microscopy, and flow cytometry platforms. Our fluorescent dyes are offered conjugated to antibodies, streptavidin, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling ...Fluorochrome Chart. Masterful Panel Design Reveals Insight ... Pacific Blue DAPI FXCycle Violet Vybrant DyeCycle Violet 530/30 VL2 Pacific Green Horizon BV510 vGFP LIVE/DEAD Fixable Aqua 586/20 VL3 Horizon BV570 Qdot 605 LIVE/DEAD Fixable Yellow 615/20 VL4 Horizon BV605 Qdot 625Reagent Selection Guide for the Attune Flow Cytometers. The Invitrogen Attune cytometers can be designed to accommodate the most common fluorophores and fluorescent proteins used in flow cytometry, to match the panels you are currently running. Multiple fluorescent proteins can be interrogated with the 4-laser version of the Attune Flow Cytometers. Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes. Whole blood was stained with either Pacific Blue™ Mouse IgG1, κ Isotype Control (Cat. No. 558120; dashed line histogram) or Pacific Blue™ Mouse Anti-Human CD3 (Cat. No. 558117; solid line histogram). Erythrocytes were lysed with Lysing Buffer (Cat. No. 555899). Hypoxyprobe Pacific Blue Kit (100 mg pimonidazole HCl plus 1 unit of Rat IgG1 monoclonal antibody conjugated to Pacific Blue fluorophore (HP-Pacific Blue)) Item# hp15-100kit $459.00Pacific Blue dye. Fluorescence SpectraViewer. Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH.Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ...Blue (488 nm) BD Horizon™ BB515 BD Horizon™ PE-CF594 PE-Cy™5 PE PE-Cy™7 FITC Alexa Fluor® 488 PerCP-Cy™5.5 PerCP Yellow/Green (561 nm) PE BD Horizon PE-CF594 ... Many factors can influence the relative fluorochrome/reagent performance on a given instrument, including laser power, PMT voltage, optical filters, ant ibody clone, and ...Our Fluorophore Equivalency Tool will help you find BioLegend’s equivalent fluorophores compared to other commercially available fluorophores. Simply select the name of a competitor's fluorophore from the dropdown menu to see BioLegend's equivalents. The tool also allows you to preview the excitation and emission spectra of our fluorophores ... from that of commonly used green fluorophores. Alexa Fluor 405: Excited by 405nm violet laser. Alexa Fluor 405 is an extremely dim dye, for that reason it should be only used for the brightest antigens. It is a derivated of the Cascade Blue dye. Alexa Fluor 405 conjugates show minimal spectral overlap with green fluorophores andBlue. BD Horizon V450. VPD450. Pacific Blue. ™. 375. Blue. Hoechst 33342. Alexa Fluor® 488. Yellow. PE. PI. Orange. PE-Texas Red®. Red. 7-AAD. PE-Cy5. PerCP.brightest fluorochromes offered by BD Life Sciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to similar excitation and emission properties, BD Horizon™ BV421 and BD Horizon™ V450 cannot be used simultaneously. • • • • • • 405 nm 450/40The amine-reactive Pacific Blue™ succinimidyl ester can be used to can be used to create blue-fluorescent bioconjugates with excitation/emission maxima ∼410/455 nm that are excitable by the 405 nm spectral line of the blue diode (violet) laser. View all Pacific Blue™ dye products.. View the Fluorophore Selection Guide. For Research Use Only.See full list on thermofisher.com In the basic 4-color panel (BV421, FITC, PE, APC), all fluorophores are pretty bright, so you don’t need to worry too much about matching marker expression level and fluorophore brightness. Note: FITC is the dimmest of those four fluorophores, and Pacific Blue and eFluor450 can be alternatives to BV421 but they are dimmer fluorophores.Pacific Blue, or systematically 3-carboxy-6,8-difluoro-7-hydroxycoumarin, is a fluorophore used in cell biology. Its excitation maximum lies at 401 nm, while its emission maximum is at 452 nm. [2] In contrast to the less acidic 7-hydroxy-3-carboxycoumarin (pKa=7.0), the high acidity of the phenol of Pacific Blue (pKa=3.7) causes its ... Pacific Orange dye is a bright orange-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 400/551 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm ...32 blue fluorophore 7-hydroxycoumarin (HC) 2 (Figure 1A, top). 33 In the course of our screening, however, we discovered several 34 structures that are not accepted by W37 point mutants. One of 35 the most interesting examples is Pacific Blue (PB), 7 a 36 fluorophore that differs from HC only in the two fluorine After staining a cell population with Pacific Blue™, annexin V and SYTOX™ AADvanced™, apoptotic cells show violet fluorescence, dead cells show red fluorescence, and live cells show little or no fluorescence. These populations are easily distinguished by a flow cytometer with the 405 nm and 488 nm lines for excitation.Fluorochrome Peak Channels UV Excited Fluors Peak Channel BUV395 UV2 BUV496 UV7 BUV563 UV9 BUV661 UV11 BUV737 UV14 BUV805 UV16 Violet Excited Fluors Peak Channel BV421 V1 Alexa 405, SuperBright 436 V2 eFluor 450, VioBlue, Pacific Blue V3 BV480 V4 eFluor 506 V5 BV510, VioGreen V7 BV570, Pacific Orange V8 BV605, SuperBright 600, Qdot 605 …When you’re shopping for a new vehicle, you want to get a great deal. When you’re selling a car, you want to get the best price. To make this happen, you have to know the vehicle’s value — what someone is willing to pay for it. Many people ...Spark Blue™ 550. Spark Blue™ 550 provides a unique option for adding an additional fluorophore to a high-parameter (>22 color) multicolor panel on spectral unmixing cytometers like the Cytek™ Aurora or SONY ID7000™ Spectral Cell Analyzer. It is excited off of the 488 nm laser and emits at 540 nm, filling the spectral gap between ...CyFlow™ CD3 Pacific Blue™. CD3 (T3, Leu4) is expressed on peripheral blood lymphocytes, thymocytes and Purkinje cells in the cerebellum; it is lost on B or NK cells. CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex.19 нояб. 2020 г. ... ... Pacific Blue and eFluor450 can be alternatives to BV421 but they are dimmer fluorophores. Fluorophore brightness is also dependent on ...This method is based on Förster resonance energy transfer (FRET) between endogenous tryptophan (Trp) residues and the coumarin-derived fluorophore Pacific Blue (PB). Tryptophan residues are frequently found in proteins near ligand-binding sites, making this approach potentially applicable to a wide range of systems.Marina Blue and Pacific Blue Dyes. Marina Blue and Pacific Blue dyes, both of ... As compared with the aminocoumarin derivatives, the Cascade Blue fluorophore ...Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ...Marina Blue and Pacific Blue dyes, both of which are based on the 6,8-difluoro-7-hydroxycoumarin fluorophore, exhibit bright blue fluorescence emission near 460 nm (Amine-reactive, ultraviolet light-excitable fluorophores for labeling proteins and nucleic acids—Table 1.12).Cytometer LASER emission Detector range Fluorochrome Names check Accuri C6 Blue 488 FL3 670+ PerCP PI (Viability) 7-AAD PerCP-Cy5.5 PerCP-eFluor710 PE-Cy7 PE-Vio770 PE-Cy5.5 FL2 565-605 PE PI (cell cycle) ... 2 449-471 Pacific Blue CFP Live dead violet Qdot 525 V450 eFluor450 1 415-425 BV421 Blue 488 5 750+ BB790 PE-Cy7 4 652-732 PerCP-Cy5.5 ...The Pacific Northwest is renowned for its stunning landscapes, diverse wildlife, and vibrant cities. While popular tourist destinations like Seattle and Portland are a must-visit, there’s a wealth of hidden gems waiting to be discovered in ...Spark Blue™ 550. Spark Blue™ 550 provides a unique option for adding an additional fluorophore to a high-parameter (>22 color) multicolor panel on spectral unmixing cytometers like the Cytek™ Aurora or SONY ID7000™ Spectral Cell Analyzer. It is excited off of the 488 nm laser and emits at 540 nm, filling the spectral gap between ... Fluorophore Selection Guide. Fluorophores enable researchers to expand the limits of their multicolor experiments in high content analysis, microscopy, and flow cytometry platforms. Our fluorescent dyes are offered conjugated to antibodies, streptavidin, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling ...Note that the standard Dapi/Hoechst filter set will not work with fluorophores that excite at 405 nm. For instance, Alexa 405 and Pacific Blue are bad choices for widefield scopes with standard Dapi/Hoechst filter sets that excite at 365 nm. Nov 19, 2020 · In the basic 4-color panel (BV421, FITC, PE, APC), all fluorophores are pretty bright, so you don’t need to worry too much about matching marker expression level and fluorophore brightness. Note: FITC is the dimmest of those four fluorophores, and Pacific Blue and eFluor450 can be alternatives to BV421 but they are dimmer fluorophores. Violet 405 450/50BV421 Pacific Blue VioBlue V450 SuperBright 436 eFluor 450 DAPI Hoechst BFP Sytox Blue Violet 405 525/50BV510 Pacific Orange V500 VioGreen eFluor 506 AmCyan CFP Violet 405 610/20BV605 eVolve 605 SuperBright 600 Violet 405 660/20BV650 eVolve 655 SuperBright 645 Violet 405 710/50BV711 SuperBright 702 Blue 488 525/50FITC AF488 ... Super Bright 436 Dye. Invitrogen eBioscience Super Bright 436 dye is a polymer-based fluorophore excited by the violet laser (405 nm) that emits signal at 436 nm with high efficacy. The technology behind the Super Bright polymer dye family makes them useful for identifying low-abundance markers on a cell surface in flow cytometry.Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ... Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ... Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ...Search life-sciences literature (Over 39 million articles, preprints and more)PE-Cyanine7 dye. Fluorescence SpectraViewer. PE-Cyanine7 is a tandem fluorophore that can be excited by a broad range of lasers including 488 nm, 532 nm, and 561 nm. Dye degradation is common if exposed to photobleaching, fixation, and higher temperatures. This is a bright dye often used in flow cytometry for low-abundance antigens.Fluorophore Selection Guide. Fluorophores enable researchers to expand the limits of their multicolor experiments in high content analysis, microscopy, and flow cytometry platforms. Our fluorescent dyes are offered conjugated to antibodies, streptavidin, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling ...brightest fluorochromes offered by BD Life Sciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to similar excitation and emission properties, BD Horizon™ BV421 and BD Horizon™ V450 cannot be used simultaneously. • • • • • • 405 nm 450/40NovaFluor Blue 660-120S. Phiton technology. 509/614. 3. 660/20. Can be used to replace PE-tandem dyes with much less cross-excitation and lower spectral spillover, allowing detection of an additional marker labeled with NovaFluor Yellow 660 dye; NovaFluor Blue 660-120S is brighter than NovaFluor 660-40S.An example is the blue fluorophore Pacific Blue, which is isosteric to 7-hydroxycoumarin, and yet not recognized by the latter’s ligase (W37V LplA) or any tryptophan 37 point mutant. Here we report the results of a structure-guided, two-residue screening matrix to discover an LplA double mutant, E20G/W37T LplA, that ligates …Pacific BlueTM 404 456 360,405,407 EviTag TM quantum dots-Lake Placid Blue 470 490 ... donor and acceptor fluorochrome molecule, placed in close proximity, allowing for energy transfer between the two. The tandem dye is excited at the excitation wavelength of the donor molecule and emits a photon atInvitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ...Fluorophore Selection Guide. Fluorophores enable researchers to expand the limits of their multicolor experiments in high content analysis, microscopy, and flow cytometry platforms. Our fluorescent dyes are offered conjugated to antibodies, streptavidin, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling ...We would like to show you a description here but the site won’t allow us.Fluorophore or Dye. Laser. Ex Max, nm. Em Max, nm. 405/488/561 ... Alexa Fluor, DyLight, Pacific Blue, and Texas Red are trademarks of Thermo Fisher Scientific ...the Indo-Pacific region o f Asia extending from Japan to New Guinea, and from the Persian Gulf to South Pacific Islands (Gobin et al. 2013; Soon and Ransangan 2016).Pacific Blue, or systematically 3-carboxy-6,8-difluoro-7-hydroxycoumarin, is a fluorophore used in cell biology. [1] Its excitation maximum lies at 401 nm, while its emission maximum is at 452 nm. [2]About Pacific Orange Pacific Orange® (Pac Orange, PacOrange) is an orange-emitting organic fluorophore that can be excited by the 405 nm Violet laser and captured with a 585/42 nm bandpass filter. Pacific Orange has an excititaion peak at 400 nm and an emission peak at 551 nm. Hypoxyprobe Pacific Blue Kit (100 mg pimonidazole HCl plus 1 unit of Rat IgG1 monoclonal antibody conjugated to Pacific Blue fluorophore (HP-Pacific Blue)) Item# hp15-100kit $459.00Cytek®’s Aurora Spectral Cytometer has a rapidly growing presence among flow cytometry research instruments. The Aurora is available with 3 or 5 laser configurations (355 nm, 405 nm, 488 nm, 561 nm and 633 nm), with the latter offering up to 64 different fluorescent parameters. This is only the theoretical limit as we continue to strive to ...Pacific Blue® (Pac Blue, PacBlue) is a blue-emitting organic fluorophore that can be excited by the 405 nm Violet laser and captured with a 450/50 nm bandpass filter. Pacific Blue has an excititaion peak at 401 nm and an emission peak at 452 nm.Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes. Whole blood was stained with either Pacific Blue™ Mouse IgG1, κ Isotype Control (Cat. No. 558120; dashed line histogram) or Pacific Blue™ Mouse Anti-Human CD3 (Cat. No. 558117; solid line histogram). Erythrocytes were lysed with Lysing Buffer (Cat. No. 555899). Pacific Blue, or systemically called 3-carboxy-6,8-difluoro-7-hydroxycoumarin, is a fluorophore used in cell biology. Its maximum excitation wavelength is 401 nm and its maximum emission wavelength is 452 nm. Even at a pH of about 7, its fluorescence is still very high. Pacific Blue is a member of the Pacific Dye Group.Fluorochrome Peak Channels Laser Center Channel Wavelength (nm) Bandwidth (nm) Wavelength Start (nm) Wavelength End (nm) vs Violet V12 V13 VI 4 428 4/1.3 ... Pacific Blue BV4SO 506 VioGreen 8V570, Pacific Orange BV605,Su ere Qdot605 BV650, Supergright 645, Qdot 655 BV71 su Qdot705 ev786, Qdot goo Blue Excited FluorsPacific Cross Vietnam brings unique expertise to the insurance industry in Vietnam. We are a leading administrator of health insurance and travel insurance focused on serving the …Are you looking for health insurance? Blue Cross insurance is one provider option that is widely available and, therefore, is likely to come up in your search. Learn more about where and how Blue Cross operates, including information on wha...An Improved Synthesis of the Pacific Blue Fluorophore and Fluorescence-based Studies of Receptor-Ligand Interactions Lee, Molly Marie ( University of Kansas , 2016-12-31 ) Early-stage drug discovery and chemical biology projects often use fluorescence-based assays to obtain information about biological interactions and cellular processes.Fluorophore Selection Guide. Fluorophores enable researchers to expand the limits of their multicolor experiments in high content analysis, microscopy, and flow cytometry platforms. Our fluorescent dyes are offered conjugated to antibodies, streptavidin, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling ...Spark Blue™ 550. Spark Blue™ 550 provides a unique option for adding an additional fluorophore to a high-parameter (>22 color) multicolor panel on spectral unmixing cytometers like the Cytek™ Aurora or SONY ID7000™ Spectral Cell Analyzer. It is excited off of the 488 nm laser and emits at 540 nm, filling the spectral gap between ... from that of commonly used green fluorophores. Alexa Fluor 405: Excited by 405nm violet laser. Alexa Fluor 405 is an extremely dim dye, for that reason it should be only used for the brightest antigens. It is a derivated of the Cascade Blue dye. Alexa Fluor 405 conjugates show minimal spectral overlap with green fluorophores and DAPI (4′,6-diamidino-2-phenylindole) DAPI (4′,6-diamidino-2-phenylindole) is a blue-fluorescent DNA stain that exhibits ~20-fold enhancement of fluorescence upon binding to AT regions of dsDNA. It is excited by the violet (405 nm) laser line and is commonly used as a nuclear counterstain in fluorescence microscopy, flow cytometry, and ...S2 Determination of the Kd of 16 for SA by Fluorescence Polarization (FP): The change in polarization of 16 was calculated by subtracting the average (n=3) polarization of the free ligand (P f), ...About Brilliant Violet 421. Brilliant™ Violet 421 (BV421) is a Violet-emitting non-tandem polymer fluorophore that can be excited by the 405 nm Violet laser and collected using a 450/50 bandpass filter. BV421 has an excitation peak at 405 nm and an emission peak at 421 nm, and is spectrally similar to Alexa Fluor™ 405 and Cascade Blue.Yellowfin tuna have yellow sides, blue backs, silver bellies and can weigh up to 450 pounds, whereas Pacific yellowtail have yellow stripes across their bodies, a yellow tail and weigh between 10 and 20 pounds when caught on a line.Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ...Spectrum [Pacific Blue] Pacific Blue is a fluorescent compound with an excitation peak at 404 nm and an emission peak at 455 nm. Other spectra of interest include: iFluor 405, iFluor 440, and iFluor 430. Pacific Blue belongs to the following categories: Amine Reactive Dyes and Probes for Conjugation. Purchase Pacific Blue here.Brilliant Violet dyes, including Brilliant Violet 421 (BV421), are a polymer dye-based technology and compatible with both spectral flow cytometry, as well as traditional flow cytometry. The violet laser has unique channels far from heavily occupied detectors allowing for larger panels. BV421 is excited by the 405 nm violet laser and emits at ... Pacific Orange and Pacific Blue antibody labeling kits enable attachment of a violet light-excitable fluorescent label on antibodies (up to 100 µg per reaction). Each kit contains enough dye for five reactions. Product Overview Documents Showing 2 of 2 Save to list Add to cart Recurring order eligibleFluorophore Selection Guide. Fluorophores enable researchers to expand the limits of their multicolor experiments in high content analysis, microscopy, and flow cytometry platforms. Our fluorescent dyes are offered conjugated to antibodies, streptavidin, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling ... Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ...Results and Discussion. To identify a more sensitive FRET acceptor for tryptophan, we examined the overlap between the emission spectrum of this amino acid (Ex. 280 nm, Em. ∼ 350 nm, ε = 5600 M –1 cm –1) and the absorbance spectrum of 6,8-difluoro-7-hydroxycoumarin, a fluorophore known as Pacific Blue (PB, 5). 17 Because of its …StarBright Blue 810. 477. 802. 4. 750LP. 750LP. A488 (ex max 495, ex max 419) With similar fluorescence properties to FITC, Alexa Fluor 488 is not compatible with FITC or GFP. However, as it is brighter and more photostable, it is a great alternative, especially for intracellular staining and in fluorescence microscopy.We have put together a comprehensive list of fluorochromes compatible with our MACSQuant Instruments. Multi-color flow cytometry and cell sorting applications require the use of a wide range of fluorochrome-conjugated antibodies and dyes. MACSQuant X, MACSQuant Analyzer 10, and MACSQuant Tyto® compatible dyes. Laser. Indo-Pacific tarpon have been listed as endangered (red listed), but data and information about these fish are still lacking. The results of a survey conducted in the field show that M. cyprinoides are still found in the waters of Lake Siombak, but not much research has been conducted concerning this species.blue fluorophore 7-hydroxycoumarin (HC)2 (Figure 1A, top)., from that of commonly used green fluorophores. Alexa Fluor 405: Excited by 405nm violet laser. Al, Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405, Fluorescence Emission Fluorochrome Color Ex-Max (nm) Analyzers Sorters Excitation L, CyFlow™ CD3 Pacific Blue™. CD3 (T3, Leu4) is expresse, Fluorochrome selection is a key step in designing multi-color antibody panels. The list of available fluorochrom, Fluorochrome Chart. Masterful Panel Design Reveals Insight ... Pacific Blue DAPI FXCycle Vi, Fluorophore Selection Guide. Fluorophores enable research, Invitrogen Molecular Probes Pacific Blue dye is a bright b, We selected 7-hydroxycoumarin as our fluorophore for this study becau, 32 blue fluorophore 7-hydroxycoumarin (HC) 2 (Figure 1A, top). , StarBright Blue 810. 477. 802. 4. 750LP. 750LP. A488 (ex , Fluorophore Selection Guide. Fluorophores enable rese, If it’s 10:00 p.m. Pacific time, it’s midnight Central time. The f, We report a new method to quantify the affinity of small mole, Fluorophore Selection Guide. Fluorophores enable researchers to exp, The LIVE/DEAD Fixable Red (615) Viability kit for , We selected 7-hydroxycoumarin as our fluorophore f.